Biodegradation of additive-free polypropylene by bacterial consortia enriched from the ocean and from the gut of Tenebrio molitor larvae.

Accumulation of highly recalcitrant PP wastes has caused a serious environmental pollution. We evaluated the biodegradation of two types of additive-free PP polymers by microbial degraders from different environments. Two bacterial consortia, designated as PP1M and PP2G, were enriched from the ocean and from the guts of Tenebrio molitor larvae. Both consortia were able to utilize each of two different additive-free PP plastics with relatively low molecular weights (low molecular weight PP powder and amorphous PP pellets) as the sole carbon source for growth. After a 30-day incubation, several plastic characterization methods, including high-temperature gel permeation chromatography, scanning electron microscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry, were used to characterize the PP samples. The bio-treated PP powder was covered with tight biofilms and extracellular secretions with significantly increased hydroxyl and carbonyl groups and slightly decreased methyl groups. This suggested that degradation and oxidation had occurred. The altered molecular weights and the increased melting enthalpy and average crystallinity of the bio-treated PP samples all suggested that both consortia preferred to depolymerize and degrade the fractions with molecular weights of ≤34 kDa and the amorphous phase fractions of the two types of PP. Furthermore, low molecular weight PP powder was more susceptible to bacterial degradation compared to amorphous PP pellets. This study provides a unique example of different types of additive-free PP degradation by different culturable bacteria from the ocean and insect guts as well as a feasibility of PP waste removal in different environments.

Degradation of polyvinyl chloride by a bacterial consortium enriched from the gut of Tenebrio molitor larvae.

Polyvinyl chloride (PVC), a carbon backbone synthetic plastic containing chlorine element, is one of six widely used plastics accounting for 10% global plastics production. PVC wastes are recalcitrant to be broken down in the environment but release harmful chlorinated compounds, causing damage to the ecosystem. Although biodegradation represents a sustainable approach for PVC reduction, virtually no efficient bacterial degraders for additive-free PVC have been reported. In addition, PVC depolymerization by Tenebrio molitor larvae was suggested to be gut microbe-dependent, but to date no additive-free PVC degraders have been isolated from insect guts. In this study, a bacterial consortium designated EF1 was newly enriched from the gut of Tenebrio molitor larvae, which was capable of utilizing additive-free PVC for its growth with the PVC-mass reduction and dechlorination of PVC. PVC films inoculated with consortium EF1 for 30 d were analyzed by diverse polymer characterization methods including atomic force microscopy, scanning electron microscope, water contact angle, time-of-flight secondary ion mass spectrometry, Fourier transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis technique, and ion chromatography. It was found that bio-treated PVC films were covered with tight biofilms with increased -OH and -CC- groups and decreased chlorine contents, and erosions and cracks were present on their surfaces. Meanwhile, the hydrophilicity of bio-treated films increased, but their thermal stability declined. Furthermore, Mw, Mn and Mz values were reduced by 17.0%, 28.5% and 16.1% using gel permeation chromatography, respectively. In addition, three medium-chain aliphatic primary alcohols and their corresponding fatty acids were identified as PVC degradation intermediates by gas chromatography-mass spectrometry. Combing all above results, it is clear that consortium EF1 is capable of efficiently degrading PVC polymer, providing a unique example for PVC degradation by gut microbiota of insects and a feasibility for the removal of PVC wastes.